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CO2 was continuously supplied, while CH4 was introduced intermittently for 1 min at constant interval of 3 min.
Treatment times up to 360 min at constant frequency (15 vibrations/s) and frequencies from 10 to 20 vibrations/s during 30 min were used for the preparation of the modified samples.
This was repeated at different times (5, 10, 15, 20 and 25 min) at constant concentration and also at different initial concentrations of crude oil (1.25, 2.5, 3.75, 5.0 and 6.75 g/100 mL of water) at constant time.
It should be noted that the change in CD spectrum gradually proceeded over 30 min at constant temperature as shown in Figure 4, while measurement of Tm was carried out at a heating rate of 1°C/min.
In short, the appropriate weights of metal nitrate and sodium carbonate solutions were added to 200 ml of deionized water simultaneously and drop-wise over a period of 30 min at constant pH (ca. 7.0) and temperature (323 K) with continuous stirring.
A solution of 1 mL of TBT in 10 mL of EtOH was added into the mixture of PSt latex and EtOH within 10 min at constant rate under vigorous agitation; the reaction was continued for another 2 h under stirring at 150 rpm.
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The synthesis of the resins (F/P 2.0, alkalinity 4.5 wt%) was studied at rising temperature (40 90 °C) for 90 min and at constant temperatures (80 °C, 90 °C, 100 °C, and 110 °C) for 120 min.
Figure 4 The change in CD spectra of the wild-type and mutant enzymes over a range of 0 30 min at a constant temperature.
COD removal was highest (68%%) around a pH of 8, reaction time around 90 min at a constant coagulant dosage of 2.5 g/l.
RSM methodology based on a two factor five level central composite design was employed with control variables of ozone concentration (0 to 7.8%w/w) and treatment time (0 to 10 min) at a constant gas flow rate.
Grape juice samples were sonicated with processing variables of amplitude level (24.4 61.0 μm) and treatment time (0 10 min) at a constant frequency of 20 kHz and pulse durations of 5 s on and 5 s off.
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