Sentence examples for min as recommended from inspiring English sources

Finally, those containing sperm extended with Botu-Crio® (B) were frozen after being cooled at 4-6°C 4-6°C0 min, as recommended by the manuforturer.

Living cells were incubated with probes directly added to the culture medium at 37 °C for 30 min as recommended by the supplier.

This calculation was performed for only those bouts of physical activity lasting continuously for ≥10 min as recommended, 3 and then for all ≥1 min bouts.

The reaction was performed in the presence of S-methionine at 30°C for 90 min as recommended by the supplier (Quick TNT; Promega, Madison, WI, USA).

Rat lenses or cultured HLECs were stained with a mixture of two probes; calcein AM and ethidium homodimer-1 (EthD) (Viability/Cytotoxicity Assay Kits; Biotium Inc., Hayward, CA, USA) for 30 min as recommended by the manufacturers.

To study genotoxic DNA damage, lymphocytes were treated with bleomycin at concentration 20 μg/ml in phosphate buffered saline (PBS), рН 7.4, for 30 min, as recommended by Schmezer et al. [ 20].

22 The proportion of children participating in ≥60 min MVPA (as recommended by international guidelines) was also calculated.

A short screening decontamination of 5 min was performed as recommended.

All tissues were frozen in liquid nitrogen within 15 min from resection as recommended by the Canadian Tumor Repository Network (www.ctrnet.ca).

Routine prophylactic intravenous injection of 5 IU oxytocin at delivery of the anterior shoulder or within 2 min of birth, as recommended in the national clinical practice guidelines issued by the French College of Gynecologists and Obstetricians [ 50].

The cell tracker CM-DiI (Invitrogen Inc., Carlsbad, CA, USA) was dissolved in DMSO (Invitrogen) and applied to passage 6 cells for 30 min at 37 °C, as recommended by the manufacturer.