Sentence examples for min as indicated from inspiring English sources

MCF-7 cells were incubated with 100 nmol/L AG1478 for 2 h followed by addition of EGF for 10 min as indicated, except for controls.

Biofilms grown for 2 days on 304L stainless steel electrodes at 37°C were treated with pre-treated LB medium or total applied current for 15 min as indicated.

Also, the relative diffraction intensity of (112) peak had no apparent change as the annealing time increased from 5 to 30 min, as indicated by the XRD patterns shown in Figure 5.

For the inhibition studies, cells were preincubated with the inhibitor agonists for 30 min as indicated.

In fact, both WT and Δrpd3 cells enter mitosis at ∼90 min, as indicated by the reappearance of the G1 peak.

LDS loading buffer 4X (Invitrogen) was added to the protein samples (40 µg) and they were then heated at 70°C for 10 min as indicated in manufacturer's instructions.

The IgE-primed RBL-2H3 cells were stimulated with 25 ng/ml antigen for 7 min or as indicated.

To evaluate whether LPS induces activation of NF-κB, nuclear protein extracts of serum-starved human chondrocytes were probed for the phosphorylated p65 NF-κB subunit after treatment with the indicated concentrations of LPS for 30 min. As indicated by western blotting analysis, LPS induced NF-κB activation in a dose-dependent manner.

NP-EGTA (5 mM) was introduced into the cell via the recording patch pipette for dialysis at least 15 to 20 min. As indicated in Figure 6, a 10-sec UV flash produced transient inhibition of EPSCs at the SC (30.5±1%, n = 7, P<0.01 and P<0.05 when compared to somatic and dendritic depolarization, respectively) and at the PP (9.4±2.4%, n = 7).

After stimulation (30 min or as indicated), samples were collected in 60  μl of SDS sample buffer (10 mM Tris-HCl buffer, pH 6.8, 10% glycerol, 2% sodium dodecylsulfate, 0.01% bromophenol blue and 5% β-mercaptoethanol), and boiled at 100 °C for 10 min.

Substrate specificity of cellobiohydrolases was determined by incubating 1 μg of Cbh1 or CelD with a, 1% slurry of cotton cellulose (Sigmacell 50), Avicel PH-101, PASC or 1% solution of CMC or 18 mM pNPCellobiose, incubated for 120 min or as indicated at 50°C and the release of reducing sugars determined with the DNS method [ 69].