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The aim of this study was to investigate whether a 20 min application of crushed ice at the knee immediately affects knee joint position sense during a small knee bend.
It allows for time-lapse examination of lysosomal Ca2+ store depletion and refilling with acute (<5 min) application of various pharmacological reagents, which has many advantages, including amenability to prolonged treatment protocols, as have been used predominantly in previous studies.
The combined readily bioaccessible amounts of arsenic and selenium in water soluble, exchangeable and NaOH fractions can be extracted from the solid phase within a 20 or 25 min application of 200 W cm−2 ultrasound energy in nanopure water for selenium and arsenic, respectively.
It is important to mention that disruption of microtubules by short-term (30 min) application of Nocodazole abolishes/reduces pain sensitization [58].
In a first set of experiments, the RRG response to acidosis was analyzed by subjecting preparations to 5 or 10 min application of aCSF 7.1).
In addition, a 15 min application of the CRFR1-specific agonist stressin-1 (STR; 70 nM) mimicked the effect of CRF, and this effect was also blocked with CP154526 (Figure 2C).
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In contrast, Ca2+ transients induced by 1 min applications of CCh showed a time dependent inhibition by La3+ (Fig. 7A,ii); late Ca2+ oscillations were inhibited and the plateau was markedly reduced, even in the first CCh application.
Treatment consisted of 5 min applications of serotonin and washing out (using supplemented L-15/ASW) at intervals of 15 min. Twenty-four and 48 hours after incubation in serotonin, time points reflecting the induction and the maintenance phase of long-term memory, respectively (15), ganglia were collected and samples were stored in cryotubes at –80°C until used for further analysis.
Objective: To compare skin surface temperature during the 20-min application of ice bag with and without damp towel wrap.
She received a single 60-min application of the topical capsaicin 8%% patch (Qutenza®) applied to the right side of her forehead, sparing the area around the eye.
Indeed, a 15-min application of CRF (40 nM) significantly increased CREB phosphorylation relative to vehicle-stimulated control neurons (Figure 1), but had no effect on total CREB staining (Figure S1).
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