Samples were taken at 0, 90, 180, 360, and 540 min and were analyzed by HPLC.
Pups were anesthetized by hypothermia by placing them on ice for 10 20 min and were immobilized on a platform fixed on a stereotaxic frame.
(a) iNKT cells were treated with Bodipy-conjugated or unconjugated palmitic acid for 2 min and were analyzed using flow cytometry or confocal microscopy.
After DRB removal, 1 × 106 cells were collected at several time points (0, 30, 60 and 90 min) and were subject to RNA purification and cDNA synthesis.
Extracts were incubated with 1% NP-40 at 4 °C for 30 min, and were centrifuged at 14,000 rpm at 4 °C for 10 min.
The standardized uptake values within the pheochromocytomas were greater than 25 by 10 min, and were 37 and 50 by 45 min in each dog.
Focus groups lasted between 90 and 120 min and were audio-recorded and transcribed verbatim.
The interviews took approximately 75 min, and were both voice recorded and transcribed.
Interviews lasted between 45 and 60 min and were audio-recorded with the participants' consent.
The suspensions were sonicated three times for 1 min and were shaken before measurements.
All the courses met once a week for 90 min and were taught in small classes.
