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The individual optic-tract fibres branch repeatedly, sending collaterals to the superior colliculus (SC), the medial interlaminar nucleus (MIN), and to one or more laminae within the dorsal lateral geniculate nucleus (LGNd).
With this rotor, the indoor HCHO concentration was reduced to the neighborhood of the WHO guideline (0.1 mg m−3) in 10 min and to an almost zero value in 90 min.
Under both conditions, the particle size of the treatment product was observed to increase progressively with reaction time; specifically, the d50 values obtained for the products of the combined granulation and carbonation treatment increased from 0.4 mm to 4 mm after 30 min and to 10 mm after 120 min.
The aim of the study is to examine the extraction parameters such as solvent concentration (0 100% ethanol (EtOH), v/v), the ratio of solid to solvent (25 50 mg/mL) and extraction time (20 60 min), and to obtain the best possible combinations of these parameters through response surface methodology (RS M.
Bacterial suspensions were diluted in 0.9 % NaCl to 10−7 after 30 min and to 10−8 at 12, 17.5 and 19 h.
During 6MWT the patients were instructed to walk as fast as possible for 6 min and to then decrease speed or interrupt the test if experiencing severe dyspnoea or any other discomfort.
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After washing, tissue was incubated with FITC-conjugated rabbit anti-goat (0.5 µg/ml; Molecular probes, Eugene, Oregon, USA) secondary antibody for 30 min. Actin cytoskeleton and nuclei were stained with rhodamine phalloidin (1unit/ml in PBS/BSA; Invitrogen, Paisley, UK) for 45 min and TO-PRO blue (Molecular Probes, Euegene, Oregon, USA) for 30 min respectively.
The temperature program was as follows: from 80°C (hold 2 min) to 280°C at 20°C/min and to 320°C at 5°C/min (hold 5 min); carrier gas, helium with constant flow 3 mL/min; injection temperature, 285°C; injection volume, 1 μL using pulsed splitless injection mode (splitless time, 2 min).
The reduction of plasma NEFA concentration led to significantly greater glucose clearance rate (1.9 vs. 1.2%/min) and to decreased glucose half-life (37 vs. 58 min), time to reach basal concentration (81 vs. 114 min) and glucose response area under the curve during 180 min of sampling [6,942 vs. 10,085 (μIU/mL) × 180 min].
GLC-MS analysis was performed on an HP-5890 GC interfaced to a mass selective detector 5970 MSD using a Supelco SP2330 capillary column (30 × 0.25 mm ID, Supelco) with the following temperature program: 60 °C for 1 min, then ramp to 170 °C at 27.5 °C/min, and to 235 °C at 4 °C/min with 2 min hold and finally at to 240 °C at 3 °C/min with 12 min hold.
GC injection temperature was 250°C and the GC oven temperature was initially 130°C for 4 min, rising to 230°C at 4°C/min and to 280°C at 20°C/min with a final hold at this temperature for 2 min. GC flow rate with helium carrier gas was 50 cm/s.
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