Sentence examples for min and resolved from inspiring English sources

Each episode lasted 1 2 min and resolved spontaneously.

Immunoprecipitates of α1-antitrypsin were boiled for 5 min and resolved by conventional Laemmli SDS 10%-PAGE.

For fractionation on SDS-PAGE, the same samples were mixed with 4× SDS-sample buffer, boiled for 10 min and resolved on SDS-PAGE followed by immunoblotting.

SDS-PAGE sample buffer was mixed with 30 µL of 3 × FLAG peptide eluate and the samples were boiled for 5 min and resolved by 8% SDS-PAGE.

Thirty micrograms of cellular protein or 50 µg homogenate of tissue protein was combined with 2X Laemmli buffer (Sigma, St Louis), boiled for 5 min and resolved by electrophoresis on a 10% polyacrylamide gel (BioRad).

After adding loading buffer, the samples were boiled for 10 min and resolved by Tris/Glycine SDS-Polyacrylamide gel electrophoresis, and the proteins were then transferred to a nitrocellulose membrane (Amersham) in the presence of 20% methanol and 0.1% SDS.

Less frequent local side effects were warmth (6%), redness (3%) and induration (3%) at the injection site, and pruritus (3%) that occurred (rather than locally at the injection site) on subject's right lower extremity and on the back 8 h after immunization, lasting 30 min and resolving spontaneously.

Aliquots (25 µl) of 2× SDS-PAGE loading buffer (without 2-mercaptoethanol) were added to each tube containing the immunoprecipitated proteins (pellet), boiled for 4 min, loaded, and resolved on 8.5% SDS gels.

The lysates were kept on ice for 10 min, centrifuged, and resolved by SDS/PAGE in a 6% polyacrylamide gel.

For nuclease protection assays, protein-DNA complexes were assembled on fluorescently labeled 135-mer ssDNA (10 min) before challenging with DNaseI (20 min), deproteinizing, and resolving DNA products by PAGE.

Lysates were sonicated for 4 s, centrifuged at 4°C for 2 min at 14,000×g, and resolved on 8 10% SDS-polyacrylamide gels.