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Catalase activity (CAT) in samples was recorded as a decline in absorbance at 240 nm for 1 min and expressed as μmol H2O2 consumed min−1 mg−1 protein [[24]].

Cell adhesion was measured at 0, 20, 40 and 60 min and expressed as increased absorbance at 570 nm [ 12].

Trx activity was calculated as the linear change in absorbance per min and expressed as a percentage of the enzyme activity of DMSO-treated control samples.

After correcting for background fluorescence results were calculated by subtracting fluorescence at time 30 from that at 0 min and expressed as relative fluorescence units (RFU).

TrxR activity was calculated as the linear change in absorbance per min and expressed as the percentage of enzyme activity of DMSO-treated control samples.

With epoch length set at 1 min and expressed as counts per minute, the accelerometers were to begin recording when the subject left our laboratory after the first day of testing.

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Similar(52)

The CL response was monitored at 37°C for 60 min, integrated and expressed in Relative Light Units as the mean of the response from duplicate wells.

{text{Foaming capacity }}({text) = frac{{left( {{text{Volume after }}- {text{Volume prior}}} right)}}{text{Volume prior}}{ times }100.For the determination of FS, foam volume changes in the graduated cylinder were recorded after 180 min of storage and expressed in percentage (Cano-Medina et al. 2011).

Spatial dynamics of the fluorescence intensity were measured before and within 60 min after microinjection and expressed as mean gray value [39].

Time spent in moderate and vigorous physical activity (MVPA) was defined based on cut-offs above 1000 counts · min-1, respectively, and expressed in hours per week [ 13, 25].

Seventeen hours later, cells were metabolically labeled, chased for 10, 60 or 120 min and ectopically expressed NHK was immunoisolated from cell lysates and separated in SDS-PAGE.

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