Sentence examples for min and equal from inspiring English sources

Samples were boiled for 5 min and equal amounts of protein (as determined using bound Coumassie blue [below]) from each sample were separated by SDS-PAGE.

Normal (AG10803) or NPC (GM03123) skin fibroblasts were washed 3 times with PBS, followed by incubation with RIPA buffer for 15 min. Lysates were centrifuged at 100,000 x g for 10 min and equal amounts of protein were analysed by immunoblot using commercially available antibodies.

The mix was incubated for 10 min, and equal amounts of mix added to each well of the quadruplicate.

Cell extracts were boiled for 5 min and equal concentrations of cell lysates were resolved on a SDS-NuPage gel.

Samples were heated at 95°C for 5 min and equal amounts of samples were loaded and resolved by 10% SDS-PAGE.

Finally, the beads were boiled in SDS-PAGE gel loading buffer at 96°C for 10 min and equal amounts were loaded on a gel followed by western blotting.

The optimal conditions were as follows: sulfuric acid equals 0.9%; time equals 30 min, and temperature equals 178°C.

To optimize solubilisation and protein release, lysates were incubated at 30°C for 30 min followed by centrifugation (4,500 × g, 2 min) and subsequently, equal volumes of supernatants tested for remaining 2,6-DMP oxidizing activity.

For detection of full length APP or APP CTF's, the lysates were spun at 1,000 g for 15 min and an equal amount whole protein homogenates were loaded for either 4 20% SDS-PAGE or 13% tris-tricine SDS-PAGE respectively and transferred onto nitrocellulose membranes for detection using AbD (Zymed).

Samples were taken from the primary culture every 20 min and an equal volume of fresh media added.

Cells were then counterstained with 0.5 μg/ml PI on ice for 5 min, and an equal volume of staining buffer was added.