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To ensure reproducibility of the measurements and counteract artifacts based on the temperature-dependency of this sensing method, all samples were allowed to cool down for 40 min and data points were measured in full culture medium at a sample temperature of 23 °C without any washing steps.
The final absorbance was noted up to 6 min and data for each assay was recorded in triplicate.
Occupation times with the Trimble GPS averaged 10 min and data were post-processed with Trimble Pathfinder Office software using nearby CORS stations.
Scans were taken every 60 min and data were logged to disk using the Beckman software.
Plates were read at different time points (1, 10, 20, 30, 60 min) and data were recorded as arbitrary fluorescence units.
Data needed to construct the concentration maps shown in this work were acquired within 1 min, and data processing can be performed within several minutes.
Similar(46)
Peptides were eluted with an acetonitrile gradient from 3 to 45% in approximately 55 min and data-dependent acquisition of tandem mass spectra was continuously repeated during the course of the analysis.
In this section, we assume that the time of simulation is 12 h, the time to live (TTL) of data is 12 min and the data generating interval follows exponential distribution within the range of 50 100 s.
Artificial data are simulated on a fine scale of 15/51 min and coarse data are extracted for P at 15 min intervals.
In Figure 5, the data of before exercise was obtained from 1 min to 2 min and the data of after exercise was obtained from 3 min to 4 min.
Sorption equilibria were established in ∼20 min and the data were well described by both Langmuir and Freundlich models.
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