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Where AC = absorbance of control at t = 0 min and AT = absorbance of the sample + DPPH at t = 16 min (Yen and Duh 1994).
Reactions were incubated at 37 °C for 45 min and at 80 °C for 20 min.
Blood samples were collected every 30 min from −30 to 210 min and at 270 min after GnRH treatment.
Tissue samples from mesenteric lymph nodes (MLNs), liver, lungs and spleen were analysed after 30 min and at 24 h.
Blood samples were taken immediately before drinking, at 20 min and at 60 min after alcohol drinking.
Immunoglobulin Y-urease was stable at 60 to 65°C for 30 min and at pH 4.0 for 7 h.
Its activity was lost at 80°C for 20 min and at pH 2 for 4 h.
Annealing was then performed at 150 and 300°C (60 min) and at 500°C (15 min).
Autoclaving treatment resulted in a decrease in total phenolic contents after holding for 90 min and at 121 °C at their native pH levels.
All impurities and internal standard omeprazole were eluted before 7.5 min and at 8.0 min the eluents were directed to waste.
Five milliliters of gel resulted in statistically significantly greater coverage immediately following insertion, within the first 30 min and at 6 h after insertion.
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CEO of Professional Science Editing for Scientists @ prosciediting.com