Sentence examples for min and additionally from inspiring English sources

Conditions for Caenorhabditis genus-specific primers were 95°C for 2 min followed by 40 cycles of 95°C for 1 min, 70°C for 0.75 min and 72°C for 2.25 min, and additionally a final extension step at 72°C for 10 min (Table  1).

The PCR used 0.5 U Taq Polymerase (Eppendorf) and 250 nM of each primer, with 2 min at 95°C followed by 30 cycles of 1 min 95°C, 1 min 50°C, 2 min 72°C and final extension at 72°C for 10 min. Total RNA was extracted as described above and additionally purified using RNAeasy columns (Qiagen).

There were only few changes to the observed significant associations of PM2.5 with average HR and 5-min SDNN after additionally adjusting for the circadian rhythm (morning, afternoon, evening, and night), but the estimated effect on HF power of HRV was diminished (from 13%to8.8.8% reduction) and became statistically nonsignificant.

The PCR program concisted of 30 cycli of 30 sec 95°C, 30 sec 55°C and 30 sec 72°C and additionally 5 min of denaturation at 95°C before the first cycle and an extension of 10 min. at 72°C after the last cycle.

The lysates were treated with 10% Nonidet P-40 and additionally incubated for 2 min on ice.

This mixture was then transferred to the Schlenk line and additionally purified for 30 min at 100 °C.

We chose a simple motor task that is highly overlearned and automatic and additionally included a 5-min training session before recordings to make sure that all participants were able to perform the task adequately.

They breathed NO2-air mixtures containing 0.5 to 8.0 ppm NO2 for up to 15 to 60 min. Additionally in nine subjects the protective action of atropine, meclastine, and orciprenaline was investigated.

This new statistical method considerably speeds up the OceanRAIN data post-processing and, additionally, introduces an objective precipitation phase probability for rain, snow and mixed-phase precipitation at 1-min resolution77.

And additionally?

Present were Cranston, DeConcini, Glenn, McCain, and additionally Riegle.