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The optimum extraction parameters were an extraction time of 40 min, an extraction pressure of 0.60 MPa, an extraction temperature of 44 °C and a raw material particle size of 0.45 mm.
Since the extraction only increases by 7%% from 42 to 60 min, an extraction time longer than 60 min was expected to be less significant.
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The best couple temperature – duration were 125 °C/57 min and 140 °C/40 min with an extraction yield of about 25%.
The maximum sum of the total peak area was acquired for an equilibrium and extraction temperature of 70°C, an equilibrium time of 60 min, and an extraction time of 30 min.
The maximum sum of the total peak area was acquired for a temperature of 70°C, an equilibrium time of 60 min, and an extraction time of 30 min (Table 2).
This new method enables stilbenes to be extracted from grape stems and canes in only 5 min, with an extraction temperature of 125 °C and 80% ethanol in water as the extraction solvent.
Triton X-100 extraction of the P1 was carried out at 4 °C for 20 min in an extraction buffer (0.5% Triton X-100, 150 mM KCl and protease inhibitors).
The order of factor effects on polysaccharides production was found to be extraction time (min, A) > ratio of solvent to solid (C) > extraction temperature (°C, B) > extraction number (D).
The results show that the effects of extraction time (min, A) and ratio of solvent to solid (C) were more significant than those of the other factors.
On the day of collection, sediment recovered from each deployment of the needle sampler was subjected to two different extractions : (1) a hot 10% HCl leach for 30 min (17) and (2) an extraction at room temperature in a N2-purged 1 M NaH2PO4 solution (pH∼5) for 24 h (18, 19).
However, even after a 90 min extraction, a significant amount of insoluble material remained, which a western blot showed contained significant amounts of VGLUT1, and thus indicating that the protein was aggregated already prior to the detergent extraction (Fig. 4).
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