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This suspension was heated to 95 °C for 1 min, allowed to slowly cool for 20 min and then cell debris was removed by centrifugation.
Prior to sensitisation, the TiO2 photo-anodes were pre-heated at 150 °C for 30 min, allowed to cool.
The resultant mixtures were warmed over a water bath at 80˚C for 20 min, allowed to cool slowly and kept at room temperature for crystallization.
Aqueous extraction of tannin colorants from tea was prepared by adding 2 and 5 g commercially available tea powder to 100 ml distilled water and the mixture was stirred, heated, held at the boil for 30 min, allowed to stand for 15 min and then filtered and used for dyeing cotton [78].
The pH and electric conductivity of the mixture were determined on days 0, 10, 20, 30 and 40. 10 g sample was added to 100 mL distilled water, mixed with magnetic stirrer for 20 min, allowed to stand for 24 h and then filtered.
The plug was heated to 100°C for 5 min, allowed to cool, and alkylated in the dark for 30 min with 75mM iodoacetamide.
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Mixture was vortexed vigorously for 3 min and allowed to stand for 30 min.
Tubes were then shaken for 10 min and allowed to stand for 15 min to complete the phase separation.
Analytes were injected for 5 min and allowed to disassociate for 10 min.
After this, a period of 120 min was allowed to elapse followed by a 120-min hyperinsulinemic hypoglycemic experimental period.
Another 20 min was allowed to obtain Ag2S hollow octahedron.
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