Morphine-induced phospho- (P- GSK3beta was reduced 5 min after reP- GSK3betan DBRs compared wash NDBreduced3 +/- 0.29 and 1.94 +/- 0.12 [P < 0.05] pg/minrog tissue, respectively).
Treatment with 1%, 2% or 4% H2 inhalation was given from 10 min before reperfusion to 60 min after reperfusion (total 70 min).
A catheter was inserted into the right femoral artery for continuous mean arterial pressure, physiological parameters and blood sampling at baseline, 5 min after occlusion and 20 min after reperfusion.
Before the aorta was clamped, and 10 min after reperfusion, blood samples from the coronary sinus were obtained to assay α-tocopherol, β-carotene, ubiquinol, and thiobarbituric acid reactive substances (TBARS).
Ventilation was in fact resumed only 15 min after reperfusion.
At 90 min after reperfusion, the 18-point Neurological Severity Score (NSS) was assessed.
MnTE-2-PyP(5+), given 5 or 90 min after reperfusion, reduced infarct size by 70-77% and had no effect on temperature.
Table 1 shows the systemic and renal hemodynamic variables: MAP, RBF, RVR, DO2, VO2, CμpO2, and MμpO2 at baseline (BL), 15 min after reperfusion (R15), and 90 min after reperfusion (R90).
Arterial blood samples (0.5 mL) were taken from the femoral artery at baseline and 15 and 90 min after reperfusion.
Experimentally, in isolated perfused rabbit hearts, the total GSH content fell by 54% after 90 min of ischaemia and by 61% 30 min after reperfusion.
This same comparison, by logistic-regression analysis, showed that the MB2/MB1 ratio discriminated between the groups 50 min after reperfusion (P less than 0.05); MB2 showed near-significance at 100 min (P less than 0.057); and CK-MB achieved significance after 200 min (P less than 0.05).
