Sentence examples for min after plating from inspiring English sources

As shown in Fig. 1A, uncaging done at 0 00 to the keratocytes that were already adaptive to the patterned ECM substrate (i.e., >30 min after plating) enabled many of them to resensitize and response to substrate guidance again by preferentially spreading and migrating along the FBN path (0 30 and 5 00).

Nonadherent cells were removed 20 min after plating by changing the culture medium to DMEM/F12 containing 2% FBS.

Cells were examined with a U Plan FL N 60×/0.9 objective (Olympus, Japan) at different time intervals starting 10 min after plating.

However, integrin β1 knockdown cells failed to continue spreading 12 16 min after plating while the proportion of spread control cells increased further.

Adhesion of UKF-NB-3, UKF-NB-3CDDP, UKF-NB-3VCR or UKF-NB-3DOX was quantified 60 min after plating the cells on to an endothelial cell monolayer.

Mock cells remained at their initial marking spots for up to 1 h, but ICSBP cells moved around and migrated out of their initial spots as early as 30 min after plating.

At time points 0, 15 and 30 min after plate opening two seedlings were blended 4x ∼10 s in ∼50 mL deionized water and leaf epidermal tissue was collected through a 100 µm nylon mesh (Millipore, Billerica, MA) and mounted on a microscope slide for imaging.

Images were collected at 1 h intervals starting 30 min after placing the plate in the IncuCyte chamber and cells were left to attach for 24 h when drug treatment was performed.

For actin dynamics experiments, MKs were treated after plating for 30 min with 1 mM cytochalasin D (gift from Benny Shilo, Weizmann Institute of Science) and subsequently the drug was washed-out by three medium changes.

Plates were examined two and six days after plating.

At 15 min after the plating, both control and NESH/Abi-3-expressing NIH3T3 cells were attached to the dishes and filopodial membrane protrusions were observed.