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At 10 min after injection, only 47% of CSΔN sporozoites are motile, however, this number is stable over time and similar to wild-type control levels at 30 min. The analysis of TRAP-VAL sporozoites showed that only 40%and20%0% of the inoculated sporozoites are motile at 10 min and 30 min after inoculation, respectively.
In parallel taken CLSM images show that bacteria already adhered to the WE 5 min after inoculation.
At high moi 5 min after inoculation, a clear layer about 50nm thick separated the particles from the cytoplasm (Figure 4A).
Static cultures were grown in hermetically sealed Schott bottles containing 80 ml of growth medium with a 30 ml headspace; cultures were flushed with a nitrogen/air mixture at 2% O2 for 10 min after inoculation and grown at 28°C.
Bars represent mean values and dashed line marks the mean value at 5 min after inoculation.
The expression of the majority of genes was highly regulated at 60 min after inoculation.
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Five mice per group were inoculated with (1) B16 cells and (2) B16 cells pretreated with 10 μg/ml Plerixafor for 30 min and, after inoculation, mice were treated twice a day with 1.25 mg/kg Plerixafor (Sigma Life Science) for 2 weeks, 5 days for week.
The starter S. thermophilus was detected by qPCR and RT-qPCR during cheese production at the industrial level, from at least 30 min after its inoculation until 81 days of ripening, supporting the possible role of this species in shaping organoleptic profiles.
Time-lapse microscopy of sporozoites, 10 min after intradermal inoculation, and CD31-labeled vascular endothelia.
Highest dispersals were seen within the first 15 min after intradermal inoculation.
Furthermore, germination could be detected in vivo as early as 20 min after subcutaneous inoculation.
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