15 min after addition, no major difference between the EDTA-treated and the PBS mock-treated samples was detectable.
Survival rates at 15, 30 and 60 min after addition of serum were determined by counting colony forming units (CFU).
The eluate was collected by centrifugation, and further incubated at 70 °C for 10 min after addition of 2-ME.
Beads were washed 4 times with NP-40 buffer, and incubated at 70 °C for 10 min after addition of 4 × LDS Sample Buffer and 2-ME.
OVA-opsonized zymosan were added to cells grown on Fluorodishes in complete medium and were washed in modified Ringer's and imaged 90 min after addition.
The toxicity of a sample could be detected by measuring the bioluminescence 30 min after addition to the freeze-dried strains.
Within 15 min after addition of 12 mM calcium chloride and 4.6% (v/v) methanol, 83 ± 4% of cw15 cells flocculated.
The donor fluorescence emission spectrum both before and 20 min after addition of the corresponding trigger (10 μM, toehold and hairpin; 20 units, ExoIII) was obtained on a Cytation 3 microplate reader (BioTek).
Cells were outlined in white and the images were a merge of the brightfield and 480/440 color-coded ratio channels 90 min after addition of FITC-OVA-zymosan.
Whole blood was incubated at 37 °C for 5 min and each sample was run for 10 min after addition of the agonist with a stir bar spinning at 1200 r.p.m.
The indicated concentrations of the peptides were added 30 min after addition of LPS.
