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at 2 s intervals for 2 min (a total of 61 frames were obtained).
The tissue/trypsin solution was shaken at 37°C for 15 min a total of three times.
Cycling parameters were: 98°C for 10 sec, 60°C for 20 sec, 72°C for 5 min a total of 20 cycles.
Images were collected every 5 min, a total z-stack of 60 to 70 μm was acquired with a slice interval of 2.5 to 3.5 μM.
After initial denaturation at 95 °C for 1 min, a total of 40 cycles (denaturation 95 °C, 5 s; annealing extension of 60 °C, 20 s) was carried out.
After preliminary perfusion with mKRB for 5 min, pregnant rats were perfused with mKRB containing 2 μM BPA-GA for 20 min. Then, mKRB without BPA-GA was perfused for 70 min (a total of 90 min).
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PCR conditions were as follow: 95°C for 5 min, followed by 95°C for 1 min, 55°C; 1 min, 72°C; 1 min, for a total of 40 cycles.
In brief, following the 90-min stabilization period, cefadroxil saline solution was infused iv at 0.3 mg/kg/min for 20 min followed by 0.15 mg/kg/min for 400 min (for a total of 420 min).
Using a cumulative stopwatch total immobility time was recorded during the last 4 min of a total 6 min video.
The total duration of immobility was recorded during the next 4 min of a total 6 min test.
The system was kept at 10 90 for 5 min and then re-equilibrated to 95 5 in 10 min for a total of 65 min run cycle.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com