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For staining 5hmC, DNA was denatured with 2 mol/L HCl for 30 min, and then neutralized with 0.1 mol/L Tris-HCl (pH 8.5) twice (10 min a time).
The reaction time is optimized for completion of the reaction, and the solution is left to stir for 15 60 min, a time beyond which no major change in the product is observed.
Due to the very short half-life of 11C (20.4 min), a time lag of at least 3 hr between scans provided sufficient decay time of radioactivity in monkeys (approximately 1/400 of the injected dose).
Due to the very short half-life of 11C (20.4 min), a time lag of at least 3 hours between the two PET scans in an individual monkey in the same day provided a sufficient decay time of radioactivity (approximately 1/400 of the injected dose).
We measured cAMP-independent phosphorylation of PDE4D at 5 min, a time point that showed sub-maximal substrate phosphorylation.
We tested this possibility after stabilizing EGFR with chloroquine and stimulating with EGF for 2, 5, or 10 min, a time range that shows maximal receptor ubiquitination.
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Remarkably, the subsequent addition of the helix B S511D mutant resulted in an increase in fluorescence within 1 min, a time-course that was beyond the resolution of our experimental set up.
The isolation and preparation of autogeneic mitochondria is rapid and purified mitochondrial are available within 30 min, a time-frame within the clinical time frame for use in surgery.
Mosquitoes are fed by AF for 15 min at a time.
Each session lasts at least 30 min at a time.
Each session lasts at least 30 min at a time. 1 = Sedentary leisure time.
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