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All the LiPs adopted secondary structure in a membrane mimicking environment.
The C-terminal fragment has the ability to adopt predominant β-sheet conformation even in the presence of detergent and membrane mimicking environment.
Biodegradable porous scaffolds can be surface engineered to provide an extracellular matrix mimicking environment for better cell adhesion and tissue in-growth.
Long term 3D cell culture was used to assess autophagy in a tissue mimicking environment in vitro.
Long term cell culture in 3-dimensional ALVETEX scaffolds (Reinnervate, Sedgefield, UK) fosters cell-cell-interactions in a tissue mimicking environment and has been described previously [ 24].
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D-MPI takes a left hand α-helical conformation in the membrane-mimicking environment.
It was found to assume amphipathic α-helical conformation in membrane-mimicking environment and exert antimicrobial activity at micromolar concentrations.
SEIRA studies of membrane proteins, however, remain challenging due to sample stability, effects of the metal surface on function, and the need for a membrane-mimicking environment.
The secondary structure of these peptides were determined in 10 mM sodiumphosphate buffer (PBS, pH 7.4) and a membrane-mimicking environment (50% 2,2,2-trifluoroethanol) by circular dichroism spectroscopy.
In the membrane-mimicking environment, D-MPI showed a negative band at 195 nm, and two positive bands near 208 and 222 nm, which was mirror symmetrical to its L-counterpart polybia-MPI.
Tissue scaffolds are essential in tissue engineering approaches as they provide a tissue-mimicking environment for cells, with the aim to promote cell proliferation, cell differentiation, and tissue regeneration.
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