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The paper shows that FEM models for both UT and DENT tests can reproduce the experimental load displacement curves quite accurately, and concludes that with a proper yield function to reflect the deformation involved in the mechanical tests, the deformation behaviour observed experimentally can be accurately mimicked using the FEM simulation.
Loss of the GTP cap, which can be mimicked using the slowly hydrolyzed analogue GMPCPP, converts the growing microtubule into a shrinking one (catastrophe).
Dehydrative stress was mimicked using the macromolecule polyethylene glycol (PEG8000), which passes with either difficulty or not at all through the cell wall [ 27], thereby reducing the extracellular free water concentration [ 28].
Interestingly, Pax5 levels were also upregulated under these conditions, and the effect of IL-7 attenuation could be mimicked using the PI3-kinase inhibitor LY294002 (Ochiai et al, 2012).
Similar(54)
However, hypoxia was mimicked using deferoxamine mesylate and the invasive phenotype was examined after 24 h hypoxia.
When the cell index reached 1.0∼1.5, they were transfected with miRNA mimics using the FuGENE HD transfection reagent (Roche).
miRNA levels were increased by transfecting cells with miR-143, miR-145 or miR-205 mimics using the Lipofectamine™ 2000 system (Invitrogen, Mulgrave, Victoria) as per the manufacturer's protocol.
We mimicked this using the simplest protocol, namely adhesion to plastic in the presence of serum.
To overcome these limitations, we generated Tocilizumab specific epitope mimics by using the phage display technology and tested whether the peptide mimics could induce similar humoral responses in mice immunized with the peptides.
The effects of genetic ablation of PI3Kγ could be mimicked largely by using the inhibitor AS-605240, specific for PI3Kγ.
We evaluated the efficacy of different designs of mimic miRNAs using the luciferase assay described in Takeda et al. [ 17] (see Additional file 1: Figure S10).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com