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Next, we used a different activation time to mimic the fertilization of the egg.
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To determine if the observed fertilization cone formation was due to sperm-egg membrane fusion during fertilization, we injected demembraned sperm chromatin into egg cortex distant from the pipette penetration site (depicted in Figure 2A) and immediately activated the eggs with SrCl2 which mimics the fertilization-induced Ca2+ oscillations to induce egg activation [46], [47], [48].
In contrast, the AtrabD2b/2c mutant plant pollen crossed with a wild-type female would mimic the mutant phenotype of decreased fertilization (wild-type female flower crossed with AtrabD2b/2c mutant pollen).
Therefore the experiments where we inject transfected CHO cells into the egg mimics the fertilization of the egg by a sperm in that an inactive PLCζ rapidly becomes active upon exposure to the egg cytoplasm.
Importantly, this enzymatically active PLCζ is capable of effecting successful embryo development when injected into mammalian eggs (see Fig. 3B), via generation of the characteristic Ca2+ oscillations that mimic the physiologic egg activation phenomenon observed at fertilization (see Fig. 3A).
Even with Lefty doses able to mimic the oep phenotype at 24 hour post-fertilization (hpf), we never observed any overexpression of rasl11b.
Ionomycin treatment is thought to mimic the intracellular calcium spike that occurs immediately in the oocyte following fertilization [30].
Zebrafish embryos were exposed to AEDs from initiation of gastrula (5.25 hours post-fertilization (hpf)) to termination of hatching (72 hpf) which mimic the mammalian teratogenic experimental design.
Zebrafish embryos were exposed to AEDs from initiation of gastrula (5.25 hours post-fertilization (hpf)) to termination of hatching (72 hpf) which mimic the exposure time of mammalian teratogenic experimental design.
He particularly likes to mimic the enemy.
We mimic the behavior around us.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com