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KVM designed the laboratory experiments, performed tiRNA sponge and mimic experiments and wrote the manuscript.
The nontargeting mimic was used as the scramble control in miRNA mimic experiments.
The direct comparison provided further justification that our mimic experiments closely mirror the infection process occurred in fields.
In this study, the hsa-miR-1 mimic and AllStar siRNA were used as positive control and negative control for miRNA mimic experiments, respectively.
Consistent with miR mimic experiments, miR-93 and miR-106b knock-down promoted a dramatic decrease in the number of GFP+ colonies.
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Our results indicate that it is not possible to mimic experiment 26 with this model.
The mimic experiment by using the same condition [ 19] besides strain further showed that L-Dab addition to medium M [ 19] also caused a reduced polymyxin E production (data not shown).
For each cell line, the data were collected from three independent experiments and compared to the control group obtained by transfection of non-targeting siRNA as negative control in miRNA inhibition experiments or miRNA inhibitor as negative control in miRNA mimic experiment.
During conventional TU counting, high bacterial colony density prevented accurate determination of particles at the lower plating dilutions (Fig. 1B), a result mimicking experiments in vivo when high concentrations of phage are found in a targeted tissue.
In hypoxia-mimic experiments, cells were incubated with 100 μM of CoCl2 under normoxic conditions.
On the other hand, artefacts may be overemphasized in the mimicking experiments because the normal substrates for the digesting enzymes are not present in these conditions.
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