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SeqMap: A tool for mapping millions of short sequences to the genome.
More recently next-generation sequencing technologies have made it possible to sequence millions of short DNA-fragments.
Next-generation sequencing (NGS) technologies provide high-throughput approaches by the rapid acquisition of thousands to millions of short nucleotide sequences.
Venter first had to activate 300 temperamental sequencing machines, at $300,000 each, in order to read the letters on millions of short fragments of DNA.
Millions of short read sequences allow both the presence and abundance of transcripts to be ascertained.
A significant feature of NGS is that it produces millions of short sequence reads for its analysis.
It is based on deep sequencing of transcripts of interests to generate millions of short reads for analysis.
Using these technologies, tens of millions of short tags (25 75 bases) can now be simultaneously sequenced at less than 1% the cost of traditional Sanger methods.
The first step in the regular analysis protocol is based on the alignment of millions of short sequences obtained from each run.
These next-generation sequencers, mainly those from Illumina and 454 Life Sciences (454), generate millions of short reads that are more error-prone than the traditional Sanger sequencing.
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