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We apply phenotypic interrogation via tag sequencing (PhITSeq) to examine millions of mutant alleles through selection and parallel sequencing.
But once a sufficient number of eggs have been correctly modified they can, after many generations, produce millions of mutant mosquitoes.
They took the HA gene from avian influenza viruses found in Vietnam and made millions of mutant versions, each of which was spliced back into a copy of the original virus.
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He and colleagues screened hundreds of thousands of mutant embryos.
Alex– Let's talk diabetes with mice So maybe mice weren't the best system for identifying circadian genes and aren't ideal for experiments that require scientists to look at hundreds and hundreds of mutant animals.
Each hgRNA can produce hundreds of mutant alleles; collectively, they can generate a unique barcode that contains the full developmental lineage of each of the ~10 billion cells in an adult mouse.
Konopka and Benzer were able to identify the period gene in the first place because working with flies allowed them to look at hundreds of mutant animals until they identified some with time-keeping defects.
Large-scale genetic screens identified thousands of mutant variants that allow in vivo dissection of developmental processes at single cell and molecular resolution.
In studying thousands of yeast mutants, Ohsumi identified 15 genes essential for autophagy.
The protocol described herein allows the screening of hundreds of Sav mutants within a reasonable time frame using CFE.
Here I describe our work developing a sequencing-based screen that uses a custom-designed barcoding scheme to simultaneously measure changes in in vivo levels of pre-mRNAs in the background of thousands of arrayed mutants.
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