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The first step of the design of the separation process was the optimisation of binding and elution conditions for the target protein on the millilitre scale.
For example, a production yield of 120 μg ml−1 for IFNα2b in yeast31 could result in multiple doses when produced at the millilitre scale, as a common formulation of IFNα2b (Intron-A, db00105) is 11.6 μg (ref. 32).
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Figure 6: Programmable protein production in a millilitre-scale table-top microbioreactor.
To overcome these barriers, we developed a platform using genetically engineered Pichia pastorisstrains designed to secrete multiple proteins on programmable cues in an integrated, benchtop, millilitre-scale microfluidic device.
To overcome these barriers, we developed a platform using genetically engineered Pichia pastoris strains designed to secrete multiple proteins on programmable cues in an integrated, benchtop, millilitre-scale microfluidic device.
To demonstrate this, we developed a protocol specifically tailored for programmable protein production with our engineered strains in an integrated, millilitre-scale table-top microbioreactor that can be operated continuously for point-of-care use in personal biomanufacturing, even with limited resources.
Furthermore, manufacturing at the volume scale of individual doses (for example, millilitres) suits requirements for point-of-care applications.
Children who presented an advanced stage of HIV infection (more than 10,000 HIV-RNA copies per millilitre of plasma) ranked poorer in OHR-QoL overall scale and in the four subscales.
Ultra scale-down technologies seek to use just millilitre quantities to enhance our understanding of the impact of the process environment as a basis for process optimisation.
Modifications in the reaction design result in preparative scale production rates of cell-free reactions and yield in milligram amounts of membrane proteins per one millilitre of reaction volume.
Normal semen contains more than 60 million sperm per millilitre.
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