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Five milliliters of each eluate were pipetted onto fibroblast cultures, incubated, and subsequently stained.
Five milliliters of each semen sample was centrifuged, and the supernatant seminal plasma was stored at −20°C.
Five milliliters of each solution was placed in a 10 mL round bottomed flask and stirring was started at 100 rpm.
Twenty milliliters of each filtrate was transferred to 50 ml glass centrifuge tubes.
Two milliliters of each of TET solution and the TET-loaded LCNP formulation was loaded separately into the dialysis bags.
Ten milliliters of each extract was filtered through a sterile 0.22-μm Millipore filter directly into 190 ml molten PDA.
Similar(34)
One milliliter of each dilution was spread on petri dishes containing the nutrient medium.
One milliliter of each logarithmic phase bacterial culture was collected via centrifugation at 6000×g for 5 min.
One milliliter of each dilution was separately placed in Petri dishes, over which 10 15 ml of Potato Dextrose Agar with 60 µg/ml of chloramphenicol (PDAC) was poured.
Twenty-five milliliter of each sample were transferred into distiller's tubes and NaOH added followed by 2 3 drops of the end-point indicator, phenolphthalein.
One milliliter of each dilution was transferred to petri dishes with the nutrient medium (TSA for S. enterica and BHA for L. monocytogenes), and after 24 h of incubation at 37°C, the number of colonies formed was enumerated.
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