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Three milliliters from each supernatant fraction was collected and evaporated under a gentle nitrogen stream.
Two milliliters from each water/glycerol mixture were stacked on top of each other, starting with the 30% mixture at the bottom of the tube.
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One milliliter from each culture was added to 20 ml LB broth and incubated for 4 h at 120 rpm.
One milliliter from each of the cultures was inoculated into a 250 mL flask, containing 50 mL of YPD medium, and incubated for 5 hours under the above conditions.
One milliliter from each tube was collected at 0, 6, 12, and 24 h and immediately heat killed at 60°C for 30 min and stored at −20°C before testing with the test strip.
Model time-kill curves were determined by plotting mean colony counts (log10 CFU per milliliter) from each model versus time.
One-milliliter liquor from each reactor was used to inoculate liquid Luria Bertani (LB) medium containing the investigated heavy metal (sterilized by filtration) in that reactor.
It is $18 for 375 milliliters from bennettlane.com.com
If the team meets its stated target of using just 11 milliliters from the standard-issue 250-milliliter fuel tank, it will achieve the hoped-for 3,300 m.p.g. and set a record.
Three milliliters from the top of the solution was used for release measurement at different intervals, and then 3 mL of fresh SBF is added into the solution to keep the volume unchanged. .
Three milliliters from the top of the solution was used for release measurement at different intervals, and then 3 mL of fresh SBF is added into the solution to keep the volume unchanged.
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