One milliliter was transferred to a volumetric tube and dried in a vacuum dryer at 40-50°C 40-50°C
Nine milliliters was transferred to a dry glass tube, and 9 mL was transferred to a titanium tube.
One milliliter aliquots were transferred into microcentrifuge tubes in duplicate periodically (0-7min).
Two milliliters of seed culture were transferred to 40 mL of fermentation medium (35 g/L lactose, 30 g/L corn steep liquor, 5 g/L (NH4 2SO4, 1 g/L KH2PO4,1 g/L, K2SO4, 10 g/L CaCO3, 2 g/L phenylacetic acid, 6 ml/L corn oil, pH 6.0) and grown at 26°C with shaking at 250 r.p.m.
Twenty-five milliliter of each sample were transferred into distiller's tubes and NaOH added followed by 2 3 drops of the end-point indicator, phenolphthalein.
Two milliliters of bacterial culture were transferred into a 2 mL reaction tube with screw cap and centrifuged at 20,000 × g for 15 s.
Five milliliters of this suspension were transferred to a plastic tube.
One-milliliter volumes of overnight test tube cultures of P. putida BW11M1 were transferred to 500 ml erlenmeyers.
Fifty microliters of this culture were transferred to tubes containing each five milliliters of YMB or PMM, and incubated with agitation at 28°C.
Thirty milliliter aliquots of urine were centrifuged at 850 g for 10 minutes and the supernatants were transferred to cryovials and immediately stored at −80°C until use.
