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All blood specimens of at least 1 milliliter were injected into an aerobic blood culture bottle (Bactec Plus 26 Aerobic; BD Microbiology Systems) and cultured on MacConkey agar, horse blood agar (at 37 degrees C, each for 24 h) and Sabourand agar (at 37 degrees C for 38 hours. The isolates were characterized using biochemical tests, bioMerieux identification kit API system (bioMerieux, France).
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A total of 10 μL lentivirus (1 × 10 particles per milliliter) was injected.
Fifty milliliters of each test product was diluted with 250 mL demineralised water, of which 125 mL was injected into the Tiny-TIM system.
Ten milliliters of the suspension was decontaminated with N-acetyl-L-cysteine sodium hydroxide (0.5%NALC-2NALC-2H) (10 ), and 0.25 mL was injected onto 2 Lowenstein-Jensen slants, l containing glycerol (0.75%) and l containing pyruvate (0.6%).
Twelve milliliters (1% final concentration) of inoculum were injected into a 1200 mL aerated (1 gas volume flow per unit of liquid volume per minute (vvm)), stirred (700 rpm) and heated (30°C) Applikon 1030 bioreactor (FT Applikon Ltd., Tewkesbury, UK) with a 2.3 L full working volume.
Fifty milliliters of Ultravist 370 (Bayer Vital Leverkusen, Germany) were injected at a flow rate of 5 ml/s in an antecubital vein followed by a saline flush of 50 ml NaCl at 5 ml/s, and a start delay of 4 s.
The right knee joints of 14 rabbits were injected with 0.1 ml of a broth culture that contained 1 × 10 colony forming units per milliliter (CFU/ml) of Mycoplasma fermentans P-140.
Two milliliters 0.5% plain bupivacaine and 25 μg fentanyl were injected through the catheter.
Fourteen knee joints and one ankle were injected with 1010 or 1011 virus particles per milliliter; knee joints received 5 mL and the ankle received 2 mL.
For bulk myofiber satellite cell activation, gastrocnemius muscles were injected with cardiotoxin 1 (Sigma) dissolved at 100 micrograms per milliliter in PBS, at 4 sites of 10 microliters each for each muscle.
They were injected (i.p).
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