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A total of 10 μL lentivirus (1 × 10 particles per milliliter) was injected.
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All blood specimens of at least 1 milliliter were injected into an aerobic blood culture bottle (Bactec Plus 26 Aerobic; BD Microbiology Systems) and cultured on MacConkey agar, horse blood agar (at 37 degrees C, each for 24 h) and Sabourand agar (at 37 degrees C for 38 hours. The isolates were characterized using biochemical tests, bioMerieux identification kit API system (bioMerieux, France).
Fifty milliliters of each test product was diluted with 250 mL demineralised water, of which 125 mL was injected into the Tiny-TIM system.
Ten milliliters of the suspension was decontaminated with N-acetyl-L-cysteine sodium hydroxide (0.5%NALC-2NALC-2H) (10 ), and 0.25 mL was injected onto 2 Lowenstein-Jensen slants, l containing glycerol (0.75%) and l containing pyruvate (0.6%).
For focal injury, to assay regeneration in vivo, 5 microliters of 0.5 milligram per milliliter CTX was injected at two sites to the middle of the tibialis anterior, and muscle harvested 5 days later.
Twenty milliliters of dye was injected to the left side and 40 ml, to the other.
Forty milliliters of contrast was injected at the speed of 20 mL/second and left for exposure for 10 12 seconds.
One milliliter of bacterial inoculum was injected into the medullary canal at the osteotomy site.
Two milliliters of rat blood was injected from the eye socket vein.
Two milliliters of this solution was injected into LC-MS.
Ten milliliters of warmed saline was injected subcutaneously for rehydration.
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