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Cells were analyzed by flow cytometry and the total cell count per milliliter was calculated.
For both phenotypes, the average number of CFU per either gram or milliliter was calculated for each strain and used for HAM.
The number of lymphocytes per milliliter was calculated from the number of PBMCs obtained per milliliter blood and the proportion of lymphocytes in the total cellular population, as determined by flow cytometry, acquiring all events.
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Relative units per milliliter were calculated for all samples using this three-point standard curve.
After incubation for 4 6 days, viral plaques were counted and the viral PFU per milliliter were calculated.
Cardiac output, measured in milliliters, is calculated by multiplying stroke volume and HR.
Stroke volume, measured in milliliters is calculated by subtracting the end diameter in systole from the end diameter in diastole.
The percent injected dose per milliliter (%ID/mL) was calculated for each of the samples and the average with the standard deviation plotted for each time point.
The total functional liver volume (milliliter liver tissue) was calculated using a VOI encircling the whole liver using the iso-contour tool with a threshold of 6 kBq/mL tissue (approximately twice the radioactivity concentration in the abdominal aorta 15 20 min after tracer injection).
Tumor blood burden (tumor cells per milliliter of blood) was calculated as the total number of tumor cells in the dish divided by the volume of blood taken.
The integrity of the library [in plaque-forming units (pfu) per milliliter (pfu/ml)] was calculated using the formula: [Number of plaques (pfu) × dilution factor/volume plated (ml)] × 1000 ml / ml.
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