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Spores of each offspring were taken from these tubes and diluted in sterile water to a concentration of 200 spores per milliliter using a hemocytometer.
The concentration of CXCL12 was calculated in picograms per milliliter using a standard curve from serial diluted CXCL12 provided in the ELISA kit.
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The volume of ejaculate was measured in milliliters using a graduated cylinder.
The inflammation was quantitated in terms of milliliters using a plethysmometer (7150 Ugo Basile) which recorded small differences in water level caused by volume displacement.
We converted optical density readings of samples to milligrams per milliliter using values obtained from a standard curve generated with serial dilutions of bovine serum albumin (0.1 1.5 mg/mL).
Conidia were harvested into 0.05 % Tween 80, filtered through glass wool to remove residual mycelia and adjusted to 10 conidia per milliliter calculated using a hemocytometer.
Measurements were expressed as mean fluorescent intensity, which was converted to pictograms per milliliter by using a set of nonlinear transforms based on standard curves created in PrismGraph 4.0 (GraphPad Software Inc., San Diego, CA, USA).
The limit of detection of the assay is 300 copies per milliliter in both plasma and urine and the limit of quantitation is 1000 copies per milliliter using the NATtrol BK Virus Linearity Panel (ZeptoMetrix).
Serum 25(OH D was measured in nanograms per milliliter using radioimmunoassay.
Serum cotinine was quantified (nanograms per milliliter) using liquid chromatography-isotope dilution tandem mass spectrometry (Bernert et al. 1997).
HOMA-IR index was calculated from the values of FPG (milligrams per deciliter) and fasting insulin (microunits per milliliter) using the following formula: (glucose × insulin)/405.
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