One milliliter of warmed, resuspended Wizard DNA Clean-Up Resin (Promega Corporation, Madison, WI) was added to the mixture and mixed by inverting for 15 minutes to allow for release of the phage DNA and binding to the resin.
Five milliliters of warm (37°C) normal saline is instilled into each nostril by pipette.
Two milliliters of warm (55°C) liquid SD agar was added per well to completely cover the SE disks and allowed to solidify.
Fifty milliliters of warm 0.9% NaCl solution was instilled into the bronchial tree five times, followed by gentle suction with a negative pressure 80 100 mm Hg using a flexible fiberoptic bronchoscope (Olympus BF-260; Olympus Optical Co. Ltd ,Tokyo, Japan).
Two milliliters of warm 1× MEM containing 1% low-melting temperature agarose (SeaPlaque Agarose; FMC BioProducts, Rockland, ME) was then added and mixed with each infection, and infections were gently poured onto a layer of cooled 1% agarose in 1× MEM to create a soft agar suspension.
(946.35 milliliters) of warm water and 3 tbsp.
(4.92 milliliters) of liquid dishwashing soap, and 1 cup (236.58 milliliters) of warm water.
In a large bowl, mix 1 tablespoon (15 milliliters) of dishwashing liquid, 1 tablespoon of white vinegar, and 2 cups (236 milliliters) of warm water.
Ten milliliters of warmed saline was injected subcutaneously for rehydration.
Dissolve 1/4 to 1/2 teaspoon (1.2 milliliters to 2.5 milliliters) of salt in an 8-ounce (237 milliliters) glass of warm water and gargle to temporarily relieve a sore or scratchy throat.
