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Distribution of activity was calculated as the percentage of injected dose per milliliter of tissue (%ID/mL).
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One milliliter of the tissue homogenate was transferred to a microfuge tube and total RNA was extracted by adding 0.2 ml chloroform.
All samples were immediately serially diluted 10-fold and then inoculated into 11-day-old embryonated chicken eggs for virus titration as computed by the Reed and Muench method with results expressed as log10 50% egg infective dose per milliliter or gram of tissue collected (EID50/mL or EID50/g) [28].
Three milliliters of the tissue homogenate was processed with a Rneasy Midi kit (QIAGEN, Inc., Valencia, CA) per manufacturer's directions.
We used blood sampled from the descending aorta at a known rate using a calibrated withdrawal pump (Harvard Apparatus; Holliston, MA, USA) for 100 sec to determine the absolute flow to tissue samples in milliliters per minute or milliliters per minute per gram of tissue.
(A) The percentage of injected dose per organ and (B) percentage of injected dose per gram of tissue or milliliter of blood, respectively.
Figure4B shows the concentration of accumulated gold nanorods in the organs as percentage of injected dose per gram of tissue or milliliter of blood.
Cells were then treated with 250 μL of culture supernatant from menstrual and mid-secretory tissue explants incubated in vitro for 24 hours (40 mg of tissue per milliliter of RPMI medium) or 0.5 or 20 ng of recombinant human IL-8 (R&D Systems) in 250 μL of medium.
Instantaneous flow values were calculated for each piece of myocardial tissue as milliliters per minute per gram of tissue.
Tissue concentrations of doxorubicin were expressed as microequivalents per milliliter of plasma or per gram of tissue.
One milliliter of Trizol was added to excised tissue, and tissue was homogenized by 30 strokes of trituration.
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