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(A and B) HCV RNA levels in serum (genome copies per milliliter of serum) and liver (genome copies per milligram of liver) from mice infected by HCV with subsequent 1 week treatments with various concentrations of PBZ.
(E and F) HCV RNA levels in serum (genome copies per milliliter of serum) and liver (genome copies per milligram of liver) from mice which PBZ treatment delayed the establishment of HCV infection in mice pretreated for 1 week before infection confirming the ability of this drug to inhibit HCV infection in vivo.
(C and D) HCV RNA levels in serum (genome copies per milliliter of serum) and liver (genome copies per milligram of liver) from mice infected by HCV with the treatments of 2 mg/kg/d PBZ for 1 week to 8 weeks.
We show that sufficient miRNAs are present in one milliliter of serum to detect miRNA expression patterns, without the need for amplification techniques.
Similarly, Tardy et al, using serum samples from HIV-infected patients positive for P24 antigen, found that none of the 17 patients with less than 50 pg of P24 antigen per milliliter of serum was positive for P24 in the Determine 4G test, while only 4 of the 9 patients with values between 50 and 400 pg/ml were positive [26].
Results are expressed in picograms per milliliter of serum.
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Twenty milliliters of serum yielded ∼20 mg of IgG Abs.
Subsequent calculation of viral loads was performed based on the ratio of the estimated viral genome copy numbers in the TaqMan assay over the amount of incorporated human genomic DNA for EMBs and PBMCs, respectively, while the calculation of viral loads in the serum was normalized to milliliters of serum volume.
Two milliliters of serum were stored at −20°C for testing at the Center for Global Health Research, Kenya Medical Research Institute KEMRI /Centers for Disease Control and Prevention CDCDC) laboratory in Kisumu (human samples), and the Central Veterinary Laboratory in Nairobi (animal samples).
Five milliliters of serum was analyzed for DDE and several PCB congeners using the capillary column gas chromatography with the electron capture method [ 37].
Three milliliters of serum were placed into an 8-mL glass vial labeled with the identification code and stored frozen at −18°C.
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