Sentence examples for milliliter of medium from inspiring English sources
HSV DNA was detected using a quantitative PCR assay, and was expressed as copies per milliliter of medium.
One milliliter of medium was then added for a further 16 h, during which cells migrated onto the plastic.
The following day, one milliliter of medium was added to each well, and the cells refed every 3 4 days for 2.5 weeks.
The growth of C3H/10T 1/2 cells treated with 5, 37, 75, and 150 microgram of HBB and FF-1 per milliliter of medium was measured at 4, 8, and 13 days following treatment.
Cells were plated at a density of 5 × 105 cells / 12 mm diameter round glass coverslip in wells of a 24-well plate in one milliliter of Medium 199 (Cellgro) supplemented with 10% fetal bovine serum.
Twenty milliliters of medium seeded with test organisms were poured into 9 cm sterile Petri dishes.
One milliliter of fresh medium plus 1 ml of infectious medium, containing either pRS-DAPK siRNA A, siRNA C, or pRS vector alone were added to 2.5 × 10 Jurkat cells/well, and the 6-well plate was centrifuged at 2300 rpm for 90 min at room temperature in a Beckman centrifuge GPH, Rotor 3.7.
By solving the quadratic regression equation using appropriate statistic methods, the optimum concentration values for obtaining 876.32 μg extracellular polysaccharide per milliliter of cultivation medium were determined: yeast extract 6.0 g l−1, fructose 11.5 g l−1, magnesium sulfate 0.5 g l−1, maltose 9.6 g l−1, zinc chloride 38.6 mg l−1 and initial pH value 5.3.
Half a milliliter of growth medium (0.5 ml) was added after seeding and changed 4 days thereafter.
The syndecan-1 content was calculated as the number of nanograms of syndecan-1 in each microgram of total protein in cell extracts or the number of nanograms of syndecan-1 in each milliliter of conditioned medium.
This functional complementation occurred without any gross rearrangements in the vector structure, and the co-infected cells produced as many as 104 infectious vector particles per milliliter of culture medium.
