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One milliliter of broth was sampled before and after the glucose feeding to determine the concentrations of glucose, ethanol, and acetate.
One milliliter of broth was centrifuged and the supernatant discarded.
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Biomass levels are expressed as grams of cell weight per milliliter of fermentation broth.
One milliliter of fermentation broth was centrifuged at 13,000 rpm for 10 min and the supernatant was transferred to a new microcentrifuge tube prior to analysis.
Fifty milliliters of LB broth was taken in sterilized 250-ml conical flasks and inoculated with 0.5 ml logarithmic-phase culture of the bacterial isolate.
Ten milliliters of culture broth was centrifuged for 5 min at 13,000 rpm.
Three milliliters of culture broth were centrifuged at 4307 g for 5 min, pellets were washed with 5 mL ddH2O, centrifuged and re-suspended in ddH2O.
One milliliter of an early logarithmic broth culture of each strain was seeded in a bottle of 50-mL nutrient broth.
One milliliter broth of yeast cells was disrupted, and β-galactosidase was detected by Pichia pastoris expression kit (Invitrogen).
Two milliliters of the cultivated broth was transferred to the growth medium containing 75 g/L glycerol, 6.7 g/L yeast nitro base without amino acids, 10 g/L yeast extract, 3 g/L ammonium sulfate, 5.9 g/L potassium dihydrogen phosphate, and 1.15 g/L dipotassium hydrogen phosphate.
Five milliliters of Luria-Bertani broth cultures of single colonies were grown overnight at 37°C with shaking at 200 rpm.
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