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One milliliter of assay buffer containing 2 μ m fluo-4 AM, 0.01% pluronic acid and 2.5 m m probenecid was added to each dish and incubated at 37 °C for 45 min. The fluo-4 solution was removed and washed twice with the assay buffer and replaced with 900 μL of assay buffer.
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Five milliliters of the assay mixture for the peroxidase activity comprised 35 μL of phosphate buffer (10 mmol−1 pH 6.0), 10 μL of sample extract (coral tissue and zooxanthellae solution of six different coral samples), 40 μL of guaiacol (25 mmol−1), and 25 μL of H2O2 (20 mmol−1).
One milliliter of Radio-Immunoprecipitation Assay (RIPA) buffer was added to a 100 mm cell culture plate.
At the end of the experiment, densities (number of colony-forming units per milliliter) of evolved populations were assayed on King's B (KB) agar plates in the presence (KB + rif) or absence (KB) of rif to distinguish total count from the antibiotic-resistant subpopulation.
Endotoxin, the active agent in the walls of gram-negative bacteria, was measured in the five samples from the five reservoirs at from 2.4 × 10 to 2.5 × 10 endotoxin units per milliliter of fluid by the Limulus assay.
The luminal flush (b) was assayed for activity per milliliter of collected fluid.
HSV DNA was detected using a quantitative PCR assay, and was expressed as copies per milliliter of medium.
The real-time and nested RT-PCR, which targeted the BNI fragment reliably, detected 10 copies of RNA in the assay corresponding to 800 RNA molecules per milliliter of specimen.
With this assay as little as 10 nanograms of amphetamine per milliliter of plasma can be accurately determined.
The assay can detect 103 to 106 copies of HCV RNA per milliliter of patient serum.
For recombinant viruses titers are determined by infected cell hybridization assays and are expressed as replication units (RU) per milliliter of virus suspension.
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