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One milliliter of ethyl acetate and 20 μL of ethyl acetate (v/v) containing a percentage of glycol solution was used to re-dissolve the concentrated filtrate before transferring the extract into 2 mL vial for quantification by GC with ECD and PFPD.

One milliliter of ethyl acetate spiked with 200 ng of D2-JA and 40 ng of C6-JA-Ile, the internal standards for JA and JA-Ile, respectively, was added to each briefly crushed leaf sample (~150 mg).

Five milliliters of copper acetate solution was added to 5 ml of the methanolic extract.

The optimum absorbance values were obtained at pH 3.5 for both drugs as shown in Figure 4. Two milliliters of 0.4 M acetate buffer were sufficient to bring the optimum pH value.

"It was two sheets of acetate cylinders," he said.

One milliliter of the suspension was evenly spread on a cellulose acetate membrane filter and excess liquid was removed as previously described [ 11, 20].

One milliliter of liquefied starch solution (1 %; w/ v) in acetate buffer (100 mM, pH 5.0) was used as the substrate.

One milliliter of the experimental solution was shaken then vortexed with 500 µL of ethyl acetate.

One milliliter of extract was mixed with methanol (3 mL), aluminium chloride (0.2 mL, 10%%), potassium acetate (0.2 mL, 1 M) and distilled water (5.6 mL) and incubated the mixture for 30 min at room temperature.

One milliliter of broth was sampled before and after the glucose feeding to determine the concentrations of glucose, ethanol, and acetate.

Prices varied, from $5.55 for55 milliliters of spearmint essential oil to $51.53 for11 milliliter of white rose oil.

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