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The results are presented as the RLU per milligram of total protein.
The results are presented as relative luminescence units (RLU) per milligram of total protein.
The vertical axis shows the concentration of the target protein in picograms per milligram of total protein (TP).
Specific activity (expressed in U.mg-1) was defined as the activity per milligram of total protein, which was determined by the Lowry protein assay (Lowry 1951).
GUS activity was determined with a fluorescence photometer by measuring the amount of 4-methylumbelliferone (4-Mu) produced under the catalysis of GUS in 1 milligram of total protein per minute.
To determine the concentration of each analyte per milligram of total hepatic protein, the mass of each analyte measured through the Bio-Plex assay was divided by the corresponding Bradford Protein Assay value for the sample.
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Three milligrams of total RNA was used to isolate mRNA by binding to oligo dT) cellulose as per the manufacturer's recommendations (FastTrack 2.0, Invitrogen).
All values are normalized to milligrams of total protein.
The chlorophyll content was measured by a spectrophotometric method, and results were expressed in milligrams of total chlorophyll per gram fresh weight of tissues [ 43].
One milligram of the total RNA from each sample was reversely transcribed to cDNA by SuperScript II reverse transcriptase (Life Technologies, Grand Island, NY, USA).
Two milligrams of the total protein was used for each electrophoresis experiment.
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