Tissue protein content (in milligram protein per sample) was assessed by a Bio-Rad Protein Assay (Bio-Rad, Hercules, CA, USA), and absorbance was measured with an EL808 microplate reader (BioTek, Winooski, VT, USA).
Specific activity was defined as a rate of total enzyme activity over protein content in milligram.
To control inflammation, the volume (in milliliters) and protein content (in milligrams per milliliter) of the exudate were determined.
If they were smokers, the average number of cigarettes smoked during pregnancy was recorded and the nicotine daily intake (NDI), obtained from the average number of cigarettes smoked per day multiplied by nicotine content (in milligrams) of each cigarette, was calculated.
Normally, the urine contains only very small amounts of protein (less than 50 milligrams per 24 hours); however, protein content in the urine is increased after exercise, in pregnancy, and in some persons when standing (orthostatic albuminuria).
The proline content in each sample was measured in milligrams per gram of fresh weight (FW) and determined using a standard curve of analytical-grade proline.
In previous studies, researchers found that the average glycoalkaloid content in baked potato peels was 20 milligrams per 100 grams of fresh weight, the upper limit considered safe.
A series of rutin concentration ranging from 25 μg/mL to 200 μg/mL was prepared in methanol and the flavonoid content in the sample was expressed as milligrams of rutin equivalent per gram of extract (mg RE/g).
Protein carbonyl content in the serum was then expressed as nanomoles per milligram protein.
Cumulative proteoglycan release (GAG in the medium) and its content in cartilages (GAG in the cartilage) were expressed as mirograms of GAG per milligram wet weight of cartilage.
Parathyroid hormone content in the grafted tissue of five patients was 179 plus or minus 118.8 ng. per milligram.
