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Using immobilized metal affinity chromatography, we purified milligram amounts of hNK1R.
The established procedure is highly efficient and if scaled up could yield milligram amounts of RNCs sufficient for crystallization experiments.
We have developed a methodology for generating milligram amounts of functional Eph tyrosine kinase receptor using the protein engineering approach of expressed protein ligation.
Milligram amounts of this huge chimeric protein (217 kDa monomer) were purified from 1 l of overexpressing E. coli cells using metal-chelate affinity chromatography.
Modifications in the reaction design result in preparative scale production rates of cell-free reactions and yield in milligram amounts of membrane proteins per one millilitre of reaction volume.
We present a convenient and low-cost method to prepare milligram amounts of completely monodisperse DNA restriction fragments in a physico-chemical laboratory setting to study (in part II) the effect of limited flexibility on the concentration dependent sedimentation velocity.
Similar(32)
In addition to biopharmaceuticals, structural genomic initiatives also require milligram (mg) amounts of protein for three-dimensional (3D) structure representations.
We present here a protocol which can be used easily to obtain milligram amount of purified rat 5-HT2c receptors.
This methodology enabled us to study the activity of the BMP-2/6 heterodimer in a variety of experiments that require sub-milligram amount of ligands.
Based on the old but by far not outdated workhorse E. coli, we can readily provide milligram-amounts of high-grade (contamination-free, monodisperse, mono-isoformic, and conformationally homogeneous) protein preparations needed in diverse biotechnological fields and for various sophisticated downstream applications.
Milligram amounts per liter of culture of A2aR and A2aR GFP were functionally expressed in S. cerevisiae, with a C-terminal deca-histidine tag.
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