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The FA composition of PBMC, plasma lipid fractions, and milk were analyzed by gas chromatography.
Samples of cows', buffalos', sheeps' and goats' milk were analyzed upon clarification and appropriate dilution for the quantification of lactose, galactose, glucose and N-acetylglucosamine (GlcNAc).
Zinc and copper in serum and breast milk were analyzed by inductively coupled plasma mass spectrometry (ICP-MS).
Serum total IgE and specific IgE against epitopes of cow's milk were analyzed by enzymatic immunoassay (Phadia 100 ThermoFisher Scientific CAP system, Rodano Milano, Italy).
Thiamine and its phosphate esters in 0.5 mL of whole blood or breast milk were analyzed using precolumn derivatization, reversed-phase liquid chromatography and fluorescence detection [ 20].
For internal quality control, pools of whole blood and milk were analyzed within each batch (n = 12) of samples giving inter-batch CV's for whole blood TDP of <7% and for TMP and thiamine in milk of <5%.
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Milk was analyzed for MUN and protein content; urine was analyzed for total N, urea, and creatinine content; feces were analyzed for total N and DM content; and blood plasma was analyzed for urea and creatinine content.
Breast milk was analyzed for naphthalene using the following method.
Milk was analyzed for Hg between birth and weaning (PND21).
Human milk was analyzed for milk lipid concentration determined by creamatocrit [ 17] and carotenoid composition.
Iron in breast milk was analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES) after microwave-heated digestion.
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