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Nonspecific protein binding was blocked using a 5% milk solution at 4°C overnight.
The membranes were sequentially blocked overnight at 4 °C with 5% nonfat dry milk solution in Tris-buffered saline (TBS).
As adults, mice lever pressed for a 0.01-cc droplet of milk solution delivered immediately or four 0.01-cc droplets delivered after a delay.
Having accurate and multidimensional knowledge of the milk solution at different stages of the process is crucial in order to maintain satisfactory control of the process.
Our HLVX+ sensitive sensor in conjunction with a differential pulse stripping voltammetry (DPSV) technique was employed for the quantification of HLVX+ in buffer solution and further for food sample via spiking HLVX+ in a commercially available milk solution.
Membranes were blocked using 5% skin milk solution in TBS.
Membranes were blocked using 5% skim milk solution in TBS.
After blocking with a 5% skim milk solution, the blot was incubated with indicated antibodies.
The membrane was blocked in 5% nonfat dry milk solution in TBST at 4°C overnight.
To prevent the dephosphorylation of proteins 100 mM NaF was added to the blocking milk solution.
The resulting membrane was blocked with a 5% milk solution (0.3% Tween-PBS) for one hour at room temperature.
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