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The spiked and blank samples passed through sample preparation as other milk samples described above.
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A novel, single step method for the determination of seven ink photo-initiators in carton packed milk samples is described.
Isotopes were then readministered and blood and milk samples collected as described above with the exception that D2O was given to pups at a dose of approximately 1 g kg−1.
The bulk milk samples were tested as described for single individual milk samples.
Goats were monitored for intra-mammary infections (particularly for S. aureus) from parturition to the day of challenge with bacteriological analysis and somatic cell scores measured on milk samples each week as described by Pisoni et al. [ 21] and Moroni et al.[ 44].
The sample sizes for humans and animals for serological studies, milk samples from cattle and goats for molecular studies, were calculated by the formula of multistage random sampling described by [ 52].
Once at the laboratory, the milk samples were aliquoted and skimmed as described [ 21].
HMOs were extracted from milk samples and reduced using the method described previously.
The Organic Analytical Toxicology Branch of the National Center for Environmental Health at the Centers for Disease Control and Prevention (Atlanta, GA) analyzed milk samples for nine PBDE congeners using previously described methods (Sjödin et al. 2004).
In this study which include routine survey in milk samples during a 9 months period, we describe mastitis-associated MRSA ST398 in Dutch dairy cows.
No ethics statement was required for the collection of blood and milk samples as well as for the epidemiological surveys described above.
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