Previous studies have addressed the identification of mycobacteria in milk samples collected from both individual and collective bulk tanks.
Finally, the DSPME-GC-μECD methodology was applied to the analysis of milk samples collected in farms of dairy cattle from NW Spain.
One objective was to evaluate 2 different rapid methods to determine bacteriological properties in a large number of cow milk samples collected under field conditions.
Bacteria were successfully cultured from 35% of composite milk samples but from none of the quarter milk samples collected using conventional or cisternal sampling techniques.
In the last 5 days of each experimental period, milk yield was recorded and milk samples collected for chemical composition and FA analysis.
Despite the small sample size, for milk samples collected from the gland cistern, overall bacterial community composition differed among bedding types.
Milk samples collected twice weekly in week 3, 6, and 9 were analyzed for fat, protein, lactose, urea nitrogen, and somatic cell count.
Milk samples collected for high-abundance protein analysis were subjected to HPLC analysis to determine the content of α-casein, β-casein, and κ-casein, as well as α-lactalbumin and β-lactoglobulin.
Subsequent PCR analyses carried out in duplicate on TMR and milk samples collected at weeks 3, 4 and 12 of the study used three DNA primer sets to establish the presence or absence of transgenic DNA.
The method was successfully applied for the determination of these compounds in human milk samples collected from volunteers lactating mothers with no known occupational exposure to these compounds who live in the province of Granada Spainn).
The study materials were bulk milk samples collected from market areas of the mentioned towns.
