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The breast milk sample was obtained from a female healthy volunteer (28-years old).
Milk samples were pooled by group and another milk sample was collected from the bulk tank.
Overall, the SSP detected 85.57% of herds in which at least one individual milk sample was positive by ELISA.
Detection of Proteus Sp : One (1 ml) of milk sample was enriched in 10 ml of Buffer peptone water aseptically and incubated at 37°c for 24 h.
A depression of the freezing temperature of a milk sample compared to a reference milk sample indicated that the milk sample was contaminated with a soluble component.
Approximately a volume of 140 mL of each milk sample was collected and conserved in sterile flask and transported to the laboratory at 4 °C.
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This milk sample is analysed as previously described.
Successful measurement in milk samples was performed.
The average osmolality of undiluted milk samples was 271 mOsmol/kg.
The overall prevalence's of Bacillus cereus in raw milk samples was 38.8%.
The probability of false-negative results on a single bulk-milk sample was (1 – 0.57) = 0.43.
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