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Exact(5)
In this process, a milk sample is also collected at every farm.
The milk sample is pretreated with acetonitrile for protein precipitation and then treated with primary secondary amine for the removal of polar impurities in the matrix.
This milk sample is analysed as previously described.
Inclusion of a marker indicating the inflammatory status of the quarter could be useful, at least if only one follow-up milk sample is taken for bacteriological examination.
Since monoclonal antibodies were raised against κ-CN, the prior removal of κ-CN from the milk sample is necessary and this has been done by adding 20% TCA to a final concentration of 8%.
Similar(55)
Milk samples were pooled by group and another milk sample was collected from the bulk tank.
The breast milk sample was obtained from a female healthy volunteer (28-years old).
Every fourth milk sample was randomly selected for antibody testing.
Each milk sample was composed of seven samples taken during the morning milking.
For each herd, a bulk milk sample was collected, together with individual milk.
If the udder quarter milk had CMT ≥ 3, an aseptic milk sample was taken.
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